7kb plasmid

 

 Abstract

The 7Kb plasmid is a small plasmid found within the species diplococcus aquaticus. This plasmid is found and isolated from grown cultures, from there the use of different techniques are used and then become linear. Then change of a portion of the plasmid will change, then will be reintroduced into the same species. 

Introduction

The sample that was taken from the gel excision is to be run on another PCR with the long amp primer sets. This was then measured and run on another Gel. THis will show how the progress of our purification is going.

Methods 

Gel excisions, Standard long Amplification PCR, flurometer, nanodrop

Results

FIgure 1: 1% gel with 1st PCR, gel excision, DNA cleanup

Figure 2: 1% gel with the DNA cleanup sample

Discussion

During this week there was the trouble oof our plasmid not showing where it was supossed to. As can be seen in figure 1 there was a gel run with almost all of our samples to show what the plasmids look like. From this there was talk as to why that would happen next week will show the gel from the missing step in figure 1. As figure 2 shows there is some kind of interaction tthat is causing the plasmid to not leave the well, this will be looked at futher to decide if there is anything that can be done.