7kb plasmid
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7kn plasmid
Introduction The 7kb plasmid is being used for an isolation, transformation, and reintroduced into a different ba terrain. The use of this plasmid will be to use a smaller plasmid to introduce gnenes easier into a larger plasmid. Methods Taking 3ml of culture in 6 different tubes. This pellett was then put through 2 washes of multibuffer and mixed with lysozyme and set to incubate in a heating block. Then this culture in lysozyme was put through the zymo zippy kit isolation. Another kit was used in the second set, the thermoscience plasmid isolation kit. Following this protocal: 3ml of culture is pelletier into a 1.5 mL epindorph tube. From here there is then added with 500 microliters of multi buffer and pipette up and down and letting incubate for 5 minutes before centrifuging for 1 minute. The adding of 500 microlitters of multi buffer is added and then pipette up and down, and centrifuge for 1 min. Adding 600 microlitters of lysozyme and pipette up an...
7kb plasmid
Intoroduction For this project we will be using the 7kb plasmid that we found in deinoccocus aquaticus plasmid map. The overall goal is to be able to isolate, transform, and reintroduce to deinococcus aquaticus. During thisnproject there will be simmilar proceadures from the liniear plasmid project. This plasmid was found during a previous project, this was proven as a proof of concept plasmid. Finding it diamerized, so in gels there is a band at 1400 and a 700 band. This plasmid can have the ability to be introduced to other bacteria as well as deinococcus.