7kb plasmid

 Abstract 

The 7kb plasmid will be separated, purified,transformed and finally reintroduced into the species of deinococcus. This will be done by plasmid isolations and pcr, or restriction enzymes.

Introduction 

During an experiment it was shown that a 25ul lysozyme solution (red and lysozyme), this lysozyme is from a 10mg/mL. The new interaction that is to work with is the binding buffer, this is to see if the oweer numbers between the first and last nanodrop nubers were tested.

Methods 

Normal plasmid isolation was done as well as adding different amounts of lysozyme was added to see the affect, results in Figure 1. This showed that the use of 25ul is thee best coause of action. Following this restriction enzyme protocal will be used. from RE there will be a gel run to see if there is any improvemeents. 

Results

Figure 1: table of nanodrop

Figure 2: 1% gel that has the nanodrops samples

 

Figure 3: 1% gel showing a restriction enzyme reaction sample

Disscusion 

During this prcedure there were a few variables within the project that were in question tto begin with. The binding buffer is one of the major concerns, this is potentally causing our plasmid to not stick to the frit and causing deminished nubers. In the next step the use of a restriction enzyme was advised because in figure 2 it shows the diamerized version of the 7kb plasmid. This tells us that the process we are using is getting the plasmid but there is some function within the plasmid that causes it to dimerize. In figure 3 this was a process of both 2-3 hours of restriction enzyme. As seen in figure 3 there is nothing, so that causeds a change in the protocal for restriction enzyme.