Linerar plasmid-6

 Ran a heat gradient, saw nothing on the gel not even primers.  This canindicate either that something went wrong inthe PCR process or the samples were mixed up when  they went into the PCR reaction. So the other samples were amplified andsent through a gel. Then an isolationof tet(E.coli plasmid, primers C&D) and Left(D.Rad plasmids A&B) fragments. Following this there was a gel extraction. Ran a different heat gradient because the previous one did not produce the correct results. Have tried a few 3 way mixes to see if there will be anyway to attach the three fragments of left tet and right into a three way assemble. So far nothing on any front.