Linerar plasmid-3
This week was; testing our protocals. With a gel from last weekwas used to test dilution of primers. There was a mix up with the protocals for hilgarth where step 3 was used insted of step 2 which is a change in touchdown protocal. Touchdown is a slow change of -0.5 per cycle, which is close to a heat gradient. Did dilutions for the hilgarth protocal of primers. We warmed a sample of both dilutedprimers and undiluted primers. Also did a gel to gel clean up to runa gel, that still had streaks. Did a gel extraction, redid hilgarth step 2.
From these gels show that there is still some genomic DNA carring over from some part of the process. This willneed to be checked to see if there is anything with the primers or the protocal. Had to use the nanodrop, the florometer was not working.
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