7kb plasmid

  Abstract

The 7Kb plasmid is a small plasmid found within the species diplococcus aquaticus. This plasmid is found and isolated from grown cultures, from there the use of different techniques are used and then become linear. Then change of a portion of the plasmid will change, then will be reintroduced into the same species. 

Introduction

The data collected from the previous week was used to plan for this week as was shown perviously, therre seems to be an interaction between something in the plasmid and the primers. As this method is reaserched there is other properties to look at, such as insertion. The insertion plasmid will be kanomyacin, the primer have already been planned. 

Methods 

Results

Figure 1: Gel 1% shows the first pcr, gel excision, DNA cleanup

Figure 2: 1% gel first pcr

Figure 3: 1% gel 2nd PCR

Discussion

The results hsow that there are some trouble with the plasmid. This was the proof that there is something happening. At this point there is nothing to see on any of the gels that are available. I think that at this point there