LPC-7

 Found that the heat gradient ran from 54*C to 34*C were the best band through the gel. While there were not as bright on the gel as we would.so there was another gel run. Then the anneling temperature for the PCR reaction was changed to 62*C from now on. Left and tet was put through a heat gradient of 52-70*C. Lead put together the mix of both master mix and of primers in the solution, adding PCR water from there.