Linerar plasmid-5

 This week was finding  the equal molar for the heat gradient, the use of C1V1=C2V2.

For left there was 33ng/μl and tet was 33ng/μl. Left needed to be 50ng and tet was 35ng. So the equation tuned out to be for tet (33ng/μl)(3μl)=x(35/6), and for left(33ng/μl)(3μl)=x(50/6). These were then used for a heat gradient.